Summary

SCGE ID10000000208
Lab IDSTS159 (mTmG; Sp_t2:Sp_c20_mTmG)
Sourcein house production
Target LocusmTmG reporter transgene
Speciestransgene
DescriptionThis sgRNA targets the mTmG transgene
Guide CompatibilitySpyCas9
Target SequenceCGAAGTTATATTAAGGGTTC
Target Sequence + PAMCGAAGTTATATTAAGGGTTCGGG
Full Guide Sequence

Sp_t2: AGCAUAGCAAGUUAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUU

Sp_c20_mTmG: CGAAGUUAUAUUAAGGGUUCGUUUUAGAGCUAUGCU

Spacer SequenceCGAAGUUAUAUUAAGGGUUC
Spacer Length20
Modifications

Sp_t2: (mA)#(mG)#(mC)#(mA)(mU)(mA)(mG)(mC)(mA)(mA)(mG)(rU)(rU)(mA)(rA)(mA)(mA)(rU)(mA)(mA)(mG)(mG)(rC)(rU)(mA)(rG)(rU)(rC)(mC)(rG)(rU)(rU)(mA)(mU)(mC)(mA)(mA)(mC)(mU)(mU)(mG)(mA)(mA)(mA)(mA)(mA)(mG)(mU)(mG)(mG)(mC)(mA)(mC)(mC)(mG)(mA)(mG)(mU)(mC)(mG)(mG)(mU)(mG)(mC)#(mU)#(mU)#(mU)

Sp_c20_mTmG: (mC)#(mG)#(mA)#(mA)(mG)(mU)(mU)(mA)(mU)(mA)(fU)(fU)(fA)(fA)(rG)#(rG)#(fG)(fU)(rU)#(mC)(mG)(rU)#(rU)#(rU)#(fU)(fA)(mG)(mA)(mG)(mC)(mU)(mA)(mU)#(mG)#(mC)#(mU)

Repeat SequenceGUUUUAGAGCUAUGCU
Anit-Repeat SequenceAGCAUAGCAAGUUAAAAU
Stemloop 1 SequenceAAGGCUAGUCCGU
Stemloop 2 SequenceAACUUGAAAAAGUG
Stemloop 3 SequenceGCACCGAGUCGGUGC

Related Editors

  • SpyCas9-3xNLS

  • SpyCas9-3xNLS

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    Projects & Experiments

    This Guide: STS159 (mTmG; Sp_t2:Sp_c20_mTmG) is being used ...
    Project Initiative Contact PI
    Delivery Systems Erik J Sontheimer, PhD
    (University of Massachusetts Medical School, Worcester)    		 NIH Report
    Animal Reporter and Testing Center Stephen A Murray, PhD
    (Jackson Laboratory)    		 NIH Report