Experiment Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain Experiment Condition Sp_c20_mTmG/Sp_t2_5-3_PS-tail Assay Description: Editing efficiency determined by immunohistochemical staining for GFP. Tissue Measured: brain Measurement Type: Editing Efficiency Record ID 15000000702 Editor SpyCas9-3xNLS Delivery System Delivery System Subtype Guide STS159 (mTmG; Sp_t2-PS:Sp_c20_mTmG) Vector Model Species Mouse Model Name B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J Application Method Intra-Striatum Application Site brain Dosage 100 µM RNP Injection Frequency once Injection Rate 500 nl/min Injection Volume 2 microliters Days post injection 1 week Editor Format RNP Antidote Id Antidote Description Antibody RRID RRID:AB_2536526 Antibody Other ID AB_2536526 Antibody Description GFP Recombinant Rabbit Monoclonal Antibody, Thermo Fisher Scientific #G10362 Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: Signal Mean 3 present and 1 absent  1 present  2 present  3 present  4 absent