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Experiment: Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain

PI:   Erik J Sontheimer, PhD 

Chemically modified crRNA and tracrRNA were injected into the intra-striatum of mTmG reporter mice and activation of GFP expression was imaged.
Editing Assay: - Editing efficiency determined by immunohistochemical staining for GFP.

Organ System Overview

Delivery Efficiency
Editing Efficiency
Target Tissue
Not Available

Analyze Data Sets Available for this Experiment
  Brain (unspecified)