Experiment Independent validation for Asokan Delivery Team: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing. Experiment Condition Saline Neg control, Male Assay Description: Editing efficiency calculated by counting fluorescent cells (RFP) compared to DAPI stained nuclei Tissue Measured: heart Cell Type : cardiac muscle cell Measurement Type: Editing Efficiency Record ID 15000001297 Editor Delivery System Delivery System Subtype Guide Vector Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Application Method intravenous, tail vein Application Site liver and heart Dosage Injection Frequency once Injection Rate Injection Volume 200 microliters Days post injection 4 weeks Editor Format Antidote Id Antidote Description Measured Values Samples Size: 2   Replicate Result (Editing Efficiency) Measurement Units: Signal Mean 2 of 2 No red cells  1 No red cells  2 No red cells