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ExperimentComparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CB promoter)
Experiment ConditionMock
Assay Description: Editing efficiency calculated by counting fluorescent cells (TdTomato) compared to DAPI stained cells.
Tissue Measured:heart
Measurement Type:Editing Efficiency
Record ID15000001014

Editor
Delivery System
Delivery System Subtype
Guide
Vector

Model SpeciesMouse
Model NameB6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J

Sample Age8 weeks
Sample SexMale

Application Methodintravenous
Application Siteliver and heart
Dosage
Injection Frequency
Injection Rate
Injection Volume
Days post injection4 weeks
Editor Format
Antidote Id
Antidote Description

Measured Values
Samples Size: 1
 
Replicate Result (Editing Efficiency)
Measurement Units: % of cells
Mean 0.0 
1 0.0 
  

Publication Title
Cross-species evolution of a highly potent AAV variant for therapeutic gene transfer and genome editing. NCBI
Cross-species evolution of a highly potent AAV variant for therapeutic gene transfer and genome editing. NCBI