Independent validation for Gao Delivery Team: Testing ssAAV5 delivered intratracheally for editing activity in lung epithelia in Ai9 mice |
scAAV5-Cre-GFP Pos Control, Ai9 - SauSpyCas9, female |
PCR detection of AAV-delivered Cas9 and GFP by agarose gel electrophoresis |
lung |
respiratory epithelial cell |
Delivery Efficiency |
15000001085 |
|
Cre recombinase |
|
|
|
scAAV5-Cre-GFP; |
|
Mouse |
B6.Cg-Gt(ROSA)26Sor^em1(CAG-tdTomato)JaHe |
|
intratracheal intubation |
lung airway epithelium |
1.7E11 viral genomes |
once |
|
40 microliters |
4 weeks |
virus in sterile saline |
|
|
|
Measured Values |
Samples Size: | 2 |
|
Mean |
2 of 2 Negative  |
1 |
Negative  |
2 |
Negative  |