Experiment Testing preparation for independent validation at The Jackson Laboratory Small Animal Testing Center Experiment Condition DNMT1-C20/T2 Control Assay Description: Editing efficiency determined by immunohistochemical staining for GFP. Tissue Measured: striatum Measurement Type: Editing Efficiency Record ID 15000001496 Editor SpyCas9-3xNLS Delivery System Delivery System Subtype Guide STS204 (DNMT1; Sp_t2:Sp_c20_Dnmt1) Vector Model Species Mouse Model Name B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J Application Method Application Site brain Dosage Injection Frequency once Injection Rate Injection Volume 3 microliters, bilateral Days post injection Editor Format RNP Antidote Id Antidote Description Antibody RRID RRID:AB_1196615 Antibody Other ID AB_1196615 Antibody Description GFP (D5.1) XP Rabbit mAb antibody, Cell Signaling Technology Measured Values Samples Size: 2   Replicate Result (Editing Efficiency) Measurement Units: Signal Mean Absent  1 Absent  2 Absent

Staining for GFP in mTmG mouse injected with control DNMT1-C0/T2 modified guide none provided