STS204 (DNMT1; Sp_t2:Sp_c20_Dnmt1)

SCGE ID	10000000286
Lab ID	STS204 (DNMT1; Sp_t2:Sp_c20_Dnmt1)
Source	in house production
Target Locus	Dnmt1
Species	mouse
Description	For endogenous locus
Guide Compatibility	SpyCas9

Target Sequence	CGGGCTGGAGCTGTTCGCGC
Target Sequence + PAM	CGGGCTGGAGCTGTTCGCGCTGG
Position	GRCm39/mm39/Chr 9:20818502-20864275

Spacer Sequence	CGGGCUGGAGCUGUUCGCGC
Spacer Length	20
Modifications	SP_T2:(MA)#(MG)#(MC)#(MA)(MU)(MA)(MG)(MC)(MA)(MA)(MG)(RU)(RU)(MA)(RA)(MA)(MA)(RU)(MA)(MA)(MG)(MG)(RC)(RU)(MA)(RG)(RU)(RC)(MC)(RG)(RU)(RU)(MA)(MU)(MC)(MA)(MA)(MC)(MU)(MU)(MG)(MA)(MA)(MA)(MA)(MA)(MG)(MU)(MG)(MG)(MC)(MA)(MC)(MC)(MG)(MA)(MG)(MU)(MC)(MG)(MG)(MU)(MG)(MC)#(MU)#(MU)#(MU); SP_C20_DNMT1: (MC)#(MG)#(MG)#(MG)(MC)(MU)(MG)(MG)(MA)(MG)(FC)(FU)(FG)(FU)(RU)#(RC)#(FG)(FC)(RG)#(MC)(MG)(RU)#(RU)#(RU)#(FU)(FA)(MG)(MA)(MG)(MC)(MU)(MA)(MU)#(MG)#(MC)#(MU)

Repeat Sequence	GUUUUAGAGCUAUGCU
Anit-Repeat Sequence	AGCAUAGCAAGUUAAAAU
Stemloop 1 Sequence	AAGGCUAGUCCGU
Stemloop 2 Sequence	AACUUGAAAAAGUG
Stemloop 3 Sequence	GCACCGAGUCGGUGC

This Guide: STS204 (DNMT1; Sp_t2:Sp_c20_Dnmt1) is being used ...

Project	Initiative	Contact PI
The Jackson Laboratory Gene Editing Testing Center (JAX-GETC) Experiments Independent validation of Sontheimer delivery platform using heavily modified guide RNAs complexed with Cas9 proteins to deliver CRISPR/Cas9 to mouse brain	Animal Reporter and Testing Center	Stephen A Murray, PhD (Jackson Laboratory)
Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors Experiments Testing preparation for independent validation at The Jackson Laboratory Small Animal Testing Center	Delivery Systems	Erik J Sontheimer, PhD (University of Massachusetts Medical School, Worcester)

Guide: STS204 (DNMT1; Sp_t2:Sp_c20_Dnmt1)