Guide: STS204 (DNMT1; Sp_t2:Sp_c20_Dnmt1)
Summary
| SCGE ID | 10000000286 |
| Lab ID | STS204 (DNMT1; Sp_t2:Sp_c20_Dnmt1) |
| Source | in house production |
| Target Locus | Dnmt1 |
| Species | mouse |
| Description | For endogenous locus |
| Guide Compatibility | SpyCas9 |
| Target Sequence | CGGGCTGGAGCTGTTCGCGC |
| Target Sequence + PAM | CGGGCTGGAGCTGTTCGCGCTGG |
| Position | GRCm39/mm39/Chr 9:20818502-20864275 |
| Spacer Sequence | CGGGCUGGAGCUGUUCGCGC |
| Spacer Length | 20 |
| Modifications | SP_T2:(MA)#(MG)#(MC)#(MA)(MU)(MA)(MG)(MC)(MA)(MA)(MG)(RU)(RU)(MA)(RA)(MA)(MA)(RU)(MA)(MA)(MG)(MG)(RC)(RU)(MA)(RG)(RU)(RC)(MC)(RG)(RU)(RU)(MA)(MU)(MC)(MA)(MA)(MC)(MU)(MU)(MG)(MA)(MA)(MA)(MA)(MA)(MG)(MU)(MG)(MG)(MC)(MA)(MC)(MC)(MG)(MA)(MG)(MU)(MC)(MG)(MG)(MU)(MG)(MC)#(MU)#(MU)#(MU); SP_C20_DNMT1: (MC)#(MG)#(MG)#(MG)(MC)(MU)(MG)(MG)(MA)(MG)(FC)(FU)(FG)(FU)(RU)#(RC)#(FG)(FC)(RG)#(MC)(MG)(RU)#(RU)#(RU)#(FU)(FA)(MG)(MA)(MG)(MC)(MU)(MA)(MU)#(MG)#(MC)#(MU) |
| Repeat Sequence | GUUUUAGAGCUAUGCU |
| Anit-Repeat Sequence | AGCAUAGCAAGUUAAAAU |
| Stemloop 1 Sequence | AAGGCUAGUCCGU |
| Stemloop 2 Sequence | AACUUGAAAAAGUG |
| Stemloop 3 Sequence | GCACCGAGUCGGUGC |
Other guides that target The same sequence
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Projects & Experiments
This Guide: STS204 (DNMT1; Sp_t2:Sp_c20_Dnmt1) is being used ...| Project | Initiative | Contact PI | |
|---|---|---|---|
| Animal Reporter and Testing Center |
Stephen A Murray, PhD
(Jackson Laboratory) |
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| Delivery Systems |
Erik J Sontheimer, PhD
(University of Massachusetts Medical School, Worcester) |
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