Experiment FUS (focused ultrasound) array validation in Ai9 mice Experiment Condition Female subjects Assay Description: Edited volume: summation of total TdTomato+ area from serial sections or lightsheet detection. 1 pair analyzed in house using IHC (immunohistochemistry) /confocal; 1 pair analyzed by The Jackson Laboratory and 2 pairs analyzed using lightsheet microscope Tissue Measured: brain Measurement Type: Editing Efficiency Record ID 15000001501 Editor SaCas9 Delivery System AAV+Focused Ultrasound Delivery System Subtype Viral vector Guide Sa_Ai9_L; Sa_Ai9_R Vector Ai9LR21-SaCas9; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Application Method IV with focused ultrasound (FUS) Application Site systemic, left brain hemisphere Dosage 2E12 vg/mouse Injection Frequency once Injection Rate Injection Volume 100 microliters Days post injection 3 weeks Editor Format AAV vector encoded Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: Signal Mean Present  1 Present