Sa_Ai9_L | SCGE Toolkit

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Summary Related Editors Projects & Experiments

SCGE ID	10000000231
Lab ID	Sa_Ai9_L
Target Locus	Ai9/Ai14 reporter transgene
Species	transgene
Description	This gRNA targets the Ai9 and related transgenes, has modified scaffold (Tabebordbar Science 2016)
Guide Compatibility	SauCas9

Target Sequence	CTCTAGAGTCGCAGATCCTC
Target Sequence + PAM	CTCTAGAGTCGCAGATCCTCTAGAGT

Full Guide Sequence	CUCUAGAGUCGCAGAUCCUCGUUUAAGUACUCUGUGCUGGAAACAGCACAGAAUCUACUUAAACAAGGCAAAAUGCCGUGUUUAUCUCGUCAACUUGUUGGCGAGAUUUUUUU
Spacer Sequence	CUCUAGAGUCGCAGAUCCUC
Spacer Length	20
Modifications	none

Ai9 SaCas9 Guide B

Publication Title
Focused ultrasound-mediated brain genome editing. NCBI

This Guide: Sa_Ai9_L is being used ...

Project	Initiative	Contact PI
Focused Ultrasound-mediated Delivery of Gene-editing Elements to the Brain for Neurodegenerative Disorders Experiments FUS (focused ultrasound) array validation in Ai9 mice	Delivery Systems	Kam W Leong, PhD (Columbia University Health Sciences)