Experiment Validating Gene Editing Reporter 10 (GER10) Mouse Model in Heterozygous Blastocysts Experiment Condition ABE Assay Description: Reporter activation by ABE repair is calculated as the percent of blasts that express Venus out of the total blasts imaged. Tissue Measured: Measurement Type: Editing Efficiency Record ID 15000003447 Editor ABE7.10 Delivery System Delivery System Subtype Guide GER10-ABE-REV Vector Model Species Mouse Model Name GER10 Heterozygous Blastocyst Application Method Electroporation Application Site Dosage Injection Frequency Injection Rate Injection Volume Days post injection 96 hours Editor Format ribonucleoprotein (RNP) Antidote Id Antidote Description Measured Values Samples Size: 0   Replicate Result (Editing Efficiency) Measurement Units: % of blasts with Venus activation Mean 35.87  1 28.95  2 50  3 26.19  4 38.33  Measurement Units: % of blasts with edits Mean 29.92  1 23.68  2 37.50  3 28.57  Measurement Units: Signal Mean # blasts analyzed  1 174 blasts analyzed for Venus  Mean # blasts analyzed  1 112 blasts anlayzed for edits

Validation of Gene Editing Reporter 10 (GER10) Mouse Model in Heterozygous Blastocysts Zygotes were electroporated with Cas9 RNP along with guides and cultured 96 hours to blastocyst stage. Blastocysts were imaged for Venus fluorescence and scored for fluorescence signal.