Experiment Inhibitory effect of Cas9 RNP alone on S10- or FSDS315-mediated GFP protein delivery to HeLa cells Experiment Condition FSD315 + Cas9 RNP Assay Description: Flow Cytometry Tissue Measured: Measurement Type: Delivery Efficiency Record ID 15000002989 Editor SpCas9 (Feldan Therapeutics) Delivery System FSD315 Delivery System Subtype Amphiphilic peptide Guide Cas9-CFTR-R553X Vector Model Species Human Model Name HeLa Application Method Application Site Dosage GFP protein (10μM) + Shuttle peptide (10μM) + Cas9 RNP (2.5 μM Cas9 and 2 μM gRNA) Injection Frequency Injection Rate Injection Volume Days post injection Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Delivery Efficiency) Measurement Units: % of fluorescent cells Mean 9.51  1 9.25  2 10.11  3 9.1  4 9.58