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ExperimentAmphiphilic Peptides Deliver Base Editor RNPs to Rhesus Monkey Airway
Experiment ConditionABE8e-Cas9 RNP
Assay Description: Editing efficiency was assessed using Next Generation Sequencing of airway epithelial cells obtained by airway brushing in two airways of the airway tree
Tissue Measured:lobar bronchus of right lung cranial lobe
Cell Type :bronchial epithelial cell
Measurement Type:Editing Efficiency
Record ID15000003007

EditorABE8e-Cas9
Delivery System
Delivery System Subtype
GuidegRNA #8
Vector

Model SpeciesRhesus macaque
Model NameMacaca mulatta (Rhesus Macaque)

Application Methodintra-tracheal instillation
Application Siteairway epithelium
Dosage2 uM gRNA, 2.5 uM Cas9
Injection Frequencyonce
Injection Raten/a
Injection Volume1 ml
Days post injection7 days
Editor Formatribonucleoprotein (RNP)
Antidote Id
Antidote Description

Measured Values
Samples Size: 1
 
Replicate Result (Editing Efficiency)
Measurement Units: % of A•T basepairs converted to G•C
Mean 0.38 
1 0.38