Contact SCGE




SCGE Consortium Home   |  About SCGE Toolkit   |  Contact Us   |  Citing the Toolkit   |  License  
ExperimentAmphiphilic Peptides Deliver Base Editor RNPs to Rhesus Monkey Airway
Experiment ConditionDRI-NLS-Cy5 (10 uM)
Assay Description: Control Rhesus monkey - Cy5 without a peptide: Lack of delivery in the absence of the shuttle peptide was examined as the absence of Cy5 positive nuclei in the airway epithelial cells of various regions of the lungs.
Tissue Measured:lower respiratory tract epithelium
Cell Type :ciliated epithelial cell
Measurement Type:Delivery Efficiency
Record ID15000003038

Editor
Delivery System
Delivery System Subtype
Guide
Vector

Model SpeciesRhesus macaque
Model NameMacaca mulatta (Rhesus Macaque)

Application Methodintra-tracheal instillation
Application Siteairway epithelium
Dosage10 uM DRI-NLS-Cy5
Injection Frequencyonce
Injection Raten/a
Injection Volume1 ml
Days post injection2 hours
Editor FormatDRI-NLS-Cy5 reporter peptide
Antidote Id
Antidote Description

Antibody RRIDRRID:AB_477585
Antibody Other IDT6793
Antibody Descriptionmouse monocolonal antibody against the acetylated α-tubulin, unconjugated, T6793, Sigma-Aldrich

Antibody RRIDRRID:AB_310759
Antibody Other ID07-623
Antibody Descriptionrabbit polyclonal antibody against club cell secretory protein, unconjugated, 07-623, EMD

Antibody RRIDRRID:AB_2717534
Antibody Other IDPA5-71680
Antibody Descriptionrabbit polyclonal antibody against the pulmonary-associated surfactant protein C (SPC), unconjugated, PA5-71680, Invitrogen

Antibody RRIDRRID:AB_2565050
Antibody Other ID905501.0
Antibody Descriptionrabbit polyclonal antibody to detect cytokeratin5+ basal cells, uncojugated, 905501, Biolegend

Measured Values
Samples Size: 1
 
Replicate Result (Delivery Efficiency)
Measurement Units: Signal
Mean 0 of 1 present 
1 absent