Experiment Amphiphilic Peptides Deliver Base Editor RNPs to Rhesus Monkey Airway Experiment Condition DRI-NLS-Cy5 (10 uM) Assay Description: Control Rhesus monkey - Cy5 without a peptide: Lack of delivery in the absence of the shuttle peptide was examined as the absence of Cy5 positive nuclei in the airway epithelial cells of various regions of the lungs. Tissue Measured: lower respiratory tract epithelium Cell Type : type II pneumocyte Measurement Type: Delivery Efficiency Record ID 15000003040 Editor Delivery System Delivery System Subtype Guide Vector Model Species Rhesus macaque Model Name Macaca mulatta (Rhesus Macaque) Application Method intra-tracheal instillation Application Site airway epithelium Dosage 10 uM DRI-NLS-Cy5 Injection Frequency once Injection Rate n/a Injection Volume 1 ml Days post injection 2 hours Editor Format DRI-NLS-Cy5 reporter peptide Antidote Id Antidote Description Antibody RRID RRID:AB_477585 Antibody Other ID T6793 Antibody Description mouse monocolonal antibody against the acetylated α-tubulin, unconjugated, T6793, Sigma-Aldrich Antibody RRID RRID:AB_310759 Antibody Other ID 07-623 Antibody Description rabbit polyclonal antibody against club cell secretory protein, unconjugated, 07-623, EMD Antibody RRID RRID:AB_2717534 Antibody Other ID PA5-71680 Antibody Description rabbit polyclonal antibody against the pulmonary-associated surfactant protein C (SPC), unconjugated, PA5-71680, Invitrogen Antibody RRID RRID:AB_2565050 Antibody Other ID 905501.0 Antibody Description rabbit polyclonal antibody to detect cytokeratin5+ basal cells, uncojugated, 905501, Biolegend Measured Values Samples Size: 1   Replicate Result (Delivery Efficiency) Measurement Units: Signal Mean 0 of 1 present  1 absent