Experiment AAV Tropism project Experiment Condition AAV3b males Assay Description: Cre activity was assessed by image quantification Tissue Measured: eye Measurement Type: Editing Efficiency Record ID 15000001740 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV3b-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 0.08  1 0.19  2 0  3 0.13  4 0  Measurement Units: GC/ug DNA Mean 151.67  1 495  2 122  3 241  4 15  5 20  6 17

AAV3b eye AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging