Experiment AAV Tropism project Experiment Condition AAV3b males Assay Description: Cre activity was assessed by image quantification Tissue Measured: gastrocnemius Measurement Type: Editing Efficiency Record ID 15000001741 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV3b-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 19.45  1 11.94  2 20.47  3 28.95  4 16.44  Measurement Units: GC/ug DNA Mean 1572  1 3311  2 1563  3 663  4 124  6 2199

AAV3b gastrocnemius AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging