Experiment AAV Tropism project Experiment Condition AAV3b males Assay Description: Cre activity was assessed by image quantification Tissue Measured: spleen Measurement Type: Editing Efficiency Record ID 15000001751 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV3b-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 0.11  1 0.02  2 0.16  3 0.13  4 0.12  Measurement Units: GC/ug DNA Mean 14727.33  1 36523  2 15257  3 11037  4 2896  5 15974  6 6677

AAV3b spleen AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging