Experiment AAV Tropism project Experiment Condition AAV3b males Assay Description: Cre activity was assessed by image quantification Tissue Measured: epididymis Measurement Type: Editing Efficiency Record ID 15000001755 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV3b-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 0.66  1 0.16  2 2.15  3 0.25  4 0.08  Measurement Units: GC/ug DNA Mean 316.4  1 508  2 223  3 18  4 56  6 777

AAV3b epididymis AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging