Experiment AAV Tropism project Experiment Condition AAV3b females Assay Description: Cre activity was assessed by image quantification Tissue Measured: adrenal gland Measurement Type: Editing Efficiency Record ID 15000001756 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV3b-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Female Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 15.34  1 16.37  2 7.84  3 7.81  4 29.34  Measurement Units: GC/ug DNA Mean 1514.2  1 3529  2 2362  3 1330  5 215  6 135

AAV3b adrenal gland AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging