Experiment AAV Tropism project Experiment Condition AAV3b females Assay Description: Cre activity was assessed by image quantification Tissue Measured: diaphragm Measurement Type: Editing Efficiency Record ID 15000001759 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV3b-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Female Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 42.82  1 40.24  2 27.94  3 38.01  4 65.08  Measurement Units: GC/ug DNA Mean 2101.67  1 1683  2 2384  3 3645  4 4802  5 73  6 23

AAV3b diaphragm AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging