Experiment AAV Tropism project Experiment Condition AAV4 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: gastrocnemius Measurement Type: Editing Efficiency Record ID 15000001781 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV4-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 15.16  1 15.08  2 20.35  3 15.38  4 9.84  Measurement Units: GC/ug DNA Mean 39546.17  1 64908  2 19878  3 58595  4 70  5 73  6 93753

AAV4 gastrocnemius AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging