Experiment AAV Tropism project Experiment Condition AAV4 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: lung Measurement Type: Editing Efficiency Record ID 15000001786 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV4-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 60.31  1 56.21  2 51.56  3 68.7  4 64.76  Measurement Units: GC/ug DNA Mean 1365676  1 430244  2 380920  3 419251  4 5530  5 2836  6 6955275

AAV4 lung AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging