Experiment AAV Tropism project Experiment Condition AAV4 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: stomach Measurement Type: Editing Efficiency Record ID 15000001792 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV4-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 6.95  1 4.03  2 6.52  3 4.77  4 12.49  Measurement Units: GC/ug DNA Mean 18216  1 31991  2 3502  3 15238  4 92  5 96  6 58377

AAV4 stomach AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging