Experiment AAV Tropism project Experiment Condition AAV4 females Assay Description: Cre activity was assessed by image quantification Tissue Measured: kidney Measurement Type: Editing Efficiency Record ID 15000001803 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV4-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Female Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 6.6  1 6.94  2 7.51  3 6.36  4 5.6  Measurement Units: GC/ug DNA Mean 31348.17  1 299  2 41330  3 62  4 30578  5 60165  6 55655

AAV4 kidney AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging