Experiment AAV Tropism project Experiment Condition AAV4 females Assay Description: Cre activity was assessed by image quantification Tissue Measured: skin of body Measurement Type: Editing Efficiency Record ID 15000001809 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV4-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Female Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 5.7  1 11.93  2 5.41  3 2.95  4 2.49  Measurement Units: GC/ug DNA Mean 8465  1 113  3 99  4 14572  6 19076

AAV4 skin of body AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging