Experiment AAV Tropism project Experiment Condition AAV4 females Assay Description: Cre activity was assessed by image quantification Tissue Measured: uterus Measurement Type: Editing Efficiency Record ID 15000001815 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV4-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Female Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 11.47  1 14.73  2 12.62  3 2.93  4 15.61  Measurement Units: GC/ug DNA Mean 688.75  2 626  3 15  4 1055  5 1059

AAV4 uterus AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging