Experiment AAV Tropism project Experiment Condition AAV5 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: kidney Measurement Type: Editing Efficiency Record ID 15000001823 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV5-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 3   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 14.76  1 16.46  2 14.37  4 13.45  Measurement Units: GC/ug DNA Mean 6633.67  1 5544  2 9477  3 7737  4 11324  5 3456  6 2264

AAV5 kidney AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging