Experiment AAV Tropism project Experiment Condition AAV5 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: small intestine Measurement Type: Editing Efficiency Record ID 15000001830 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV5-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 3   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 5.39  1 6.79  2 8.26  4 1.13  Measurement Units: GC/ug DNA Mean 1417.5  1 1280  2 1656  5 2732  6 2

AAV5 small intestine AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging