Experiment AAV Tropism project Experiment Condition AAV5 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: stomach Measurement Type: Editing Efficiency Record ID 15000001832 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV5-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 3   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 9.17  1 11.48  2 14.43  4 1.59  Measurement Units: GC/ug DNA Mean 2686.67  1 1458  2 5011  3 2673  4 3637  5 3156  6 185

AAV5 stomach AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging