Experiment AAV Tropism project Experiment Condition AAV5 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: epididymis Measurement Type: Editing Efficiency Record ID 15000001835 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV5-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 3   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 4.29  1 2.01  2 6.67  4 4.19  Measurement Units: GC/ug DNA Mean 6174  1 9617  2 10389  3 4368  4 4425  5 7493  6 752

AAV5 epididymis AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging