Experiment AAV Tropism project Experiment Condition AAV5 females Assay Description: Cre activity was assessed by image quantification Tissue Measured: eye Measurement Type: Editing Efficiency Record ID 15000001840 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV5-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Sex Female Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 6.91  1 8.55  2 6.72  3 4.83  4 7.55  Measurement Units: GC/ug DNA Mean 4626  2 3636  3 907  4 2373  5 15335  6 879

AAV5 eye AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging