Experiment AAV Tropism project Experiment Condition AAV6 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: adrenal gland Measurement Type: Editing Efficiency Record ID 15000001856 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV6-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 75.09  1 74.55  2 94.07  3 67.54  4 64.18  Measurement Units: GC/ug DNA Mean 328546.5  1 444410  2 417428  3 207789  4 289270  5 37534  6 574848

AAV6 adrenal gland AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging