Experiment AAV Tropism project Experiment Condition AAV6 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: eye Measurement Type: Editing Efficiency Record ID 15000001860 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV6-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 15.48  1 16.74  2 27.18  3 10.58  4 7.4  Measurement Units: GC/ug DNA Mean 2671.67  1 1905  2 8396  3 3383  4 1112  5 24  6 1210

AAV6 eye AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging