Experiment AAV Tropism project Experiment Condition AAV6 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: spleen Measurement Type: Editing Efficiency Record ID 15000001871 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV6-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 26.08  1 25.97  2 23.26  3 26.28  4 28.79  Measurement Units: GC/ug DNA Mean 6171.83  1 8233  2 2610  3 2484  4 10646  5 3599  6 9459

AAV6 spleen AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging