Experiment AAV Tropism project Experiment Condition AAV6 females Assay Description: Cre activity was assessed by image quantification Tissue Measured: large intestine Measurement Type: Editing Efficiency Record ID 15000001884 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV6-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Female Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 6.02  1 6.2  2 4.47  3 7.85  4 5.54  Measurement Units: GC/ug DNA Mean 614.67  1 898  2 929  3 884  4 486  5 349  6 142

AAV6 large intestine AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging