Experiment AAV Tropism project Experiment Condition AAV6 females Assay Description: Cre activity was assessed by image quantification Tissue Measured: lung Measurement Type: Editing Efficiency Record ID 15000001886 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV6-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 32.41  1 24.13  2 29.6  3 23.41  4 52.51  Measurement Units: GC/ug DNA Mean 6927  1 11897  2 8115  3 11639  4 6829  5 2780  6 302

AAV6 lung AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging