Experiment AAV Tropism project Experiment Condition AAV7 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: adrenal gland Measurement Type: Editing Efficiency Record ID 15000001896 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV7-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 98.94  1 98.31  2 98.62  3 99.68  4 99.13  Measurement Units: GC/ug DNA Mean 1318652.5  1 500661  2 1441669  3 612656  4 4255859  5 823821  6 277249

AAV7 adrenal gland AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging