Experiment AAV Tropism project Experiment Condition AAV7 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: heart Measurement Type: Editing Efficiency Record ID 15000001902 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV7-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 99.02  1 98.78  2 98.55  3 99.19  4 99.54  Measurement Units: GC/ug DNA Mean 146512.5  1 126383  2 170264  3 131074  4 149803  5 100055  6 201496

AAV7 heart AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging