Experiment AAV Tropism project Experiment Condition AAV7 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: large intestine Measurement Type: Editing Efficiency Record ID 15000001904 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV7-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 29.05  1 34.14  2 34.83  3 28.96  4 18.26  Measurement Units: GC/ug DNA Mean 1828.5  1 1334  2 1709  3 3357  4 1819  5 1534  6 1218

AAV7 large intestine AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging