Experiment AAV Tropism project Experiment Condition AAV7 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: lymph node Measurement Type: Editing Efficiency Record ID 15000001907 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV7-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 13.23  1 15.36  2 11.09  3 15.18  4 11.3  Measurement Units: GC/ug DNA Mean 27000.83  1 39112  2 35040  3 53001  4 13791  5 15177  6 5884

AAV7 lymph node AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging