Experiment AAV Tropism project Experiment Condition AAV7 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: testis Measurement Type: Editing Efficiency Record ID 15000001914 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV7-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 7.42  1 7.22  2 7.18  3 7.75  4 7.53  Measurement Units: GC/ug DNA Mean 1463  1 873  2 1047  3 606  4 2811  5 674  6 2767

AAV7 testis AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging