Experiment AAV Tropism project Experiment Condition AAV7 females Assay Description: Cre activity was assessed by image quantification Tissue Measured: lung Measurement Type: Editing Efficiency Record ID 15000001926 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV7-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Female Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 35.61  1 17.06  2 33.46  3 51.7  4 40.2  Measurement Units: GC/ug DNA Mean 41791.83  1 54059  2 110661  3 22349  4 17924  5 22494  6 23264

AAV7 lung AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging