Experiment AAV Tropism project Experiment Condition AAV8 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: urinary bladder Measurement Type: Editing Efficiency Record ID 15000001937 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV8-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 30.54  1 26.13  2 55.65  3 7.56  4 32.82  Measurement Units: GC/ug DNA Mean 60667.6  1 96586  2 66771  3 27910  4 106509  6 5562

AAV8 urinary bladder AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging