Experiment AAV Tropism project Experiment Condition AAV8 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: heart Measurement Type: Editing Efficiency Record ID 15000001942 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV8-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 79.43  1 99.48  2 95.84  3 25.39  4 97.02  Measurement Units: GC/ug DNA Mean 504394.83  1 1589301  2 254634  3 567676  4 71188  5 408719  6 134851

AAV8 heart AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging