Experiment AAV Tropism project Experiment Condition AAV8 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: kidney Measurement Type: Editing Efficiency Record ID 15000001943 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV8-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 10.93  1 11.19  2 14.96  3 4.82  4 12.73  Measurement Units: GC/ug DNA Mean 67192.33  1 81766  2 65556  3 81647  4 118354  5 50730  6 5101

AAV8 kidney AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging