Experiment AAV Tropism project Experiment Condition AAV8 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: lung Measurement Type: Editing Efficiency Record ID 15000001946 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV8-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 53.89  1 69.42  2 87.62  3 12.22  4 46.29  Measurement Units: GC/ug DNA Mean 57680.83  1 51152  2 43398  3 63194  4 79623  5 99314  6 9404

AAV8 lung AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging