Experiment AAV Tropism project Experiment Condition AAV8 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: spleen Measurement Type: Editing Efficiency Record ID 15000001951 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV8-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Sex Male Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 16.08  1 16.31  2 15.54  3 13.4  4 19.07  Measurement Units: GC/ug DNA Mean 22935.8  1 14255  2 17384  3 26094  4 50522  5 6424

AAV8 spleen AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging