Experiment AAV Tropism project Experiment Condition AAV8 females Assay Description: Cre activity was assessed by image quantification Tissue Measured: urinary bladder Measurement Type: Editing Efficiency Record ID 15000001957 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV8-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Female Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 51.06  1 54.21  2 53  3 60.24  4 36.79  Measurement Units: GC/ug DNA Mean 147662.33  1 107880  2 131740  3 86368  4 413855  5 45027  6 101104

AAV8 urinary bladder AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging