Experiment AAV Tropism project Experiment Condition AAV8 females Assay Description: Cre activity was assessed by image quantification Tissue Measured: brain Measurement Type: Editing Efficiency Record ID 15000001958 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV8-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 6 weeks Sample Sex Female Application Method intravenous Application Site systemic Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 7.9  1 6.22  2 3.27  3 15.3  4 6.79  Measurement Units: GC/ug DNA Mean 25403  1 10207  2 65936  3 27010  4 7790  5 16019  6 25456

AAV8 brain AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging